Go Back  Bracket Racing > General > General Forum
Reload this Page >

Interesting Twitter Posts Regarding Inhibitors

General Forum Anything goes in this forum.

Interesting Twitter Posts Regarding Inhibitors

Old 05-16-2014, 01:37 AM
Senior Member
Thread Starter
Join Date: Jan 2014
Posts: 200
Interesting Twitter Posts Regarding Inhibitors

To discover the failure of EGFR inhibitors to block proliferation in PTENmt glioma cells, we seemed for signaling intermediates whose activation correlated with the efficacy of EGFR blockade from proliferation in tumor derived mobile strains. These data confirmed that phosphorylation of mTOR and its downstream targets SK and rpS ended up sturdy biomarkers for the skill of EGFR inhibitors to block proliferation of glioma cells. The capability of EGFR inhibitors to block Akt phosphorylation, on the other hand, correlated badly with reaction to treatment. Making use of both equally gain and decline of kinase inhibitor function approaches, we showed that Akt exercise did not correlate with activation of mTOR or with proliferation. Somewhat, we identified PKC as vital to signaling involving EGFR and mTOR in two PTENwt glioma mobile lines. We also present data from key tumor specimens that the abundances of EGFR, p PKC, and p rpS have been strongly aligned, but correlated improperly with the abundance of p Akt. Ultimately, we present that pharmacological inhibition of PKC blocked proliferation even in PTENmt glioma, where inhibition of EGFR experienced no impact. Although the BIM I inhibitor used was not particular for PKC, this agent efficiently blocked the PKC substrate p MARCKS. In addition, BIM I induced arrest at G in PTENwt cells and at G in PTENmt cells. If the antiproliferative effects of this compound were nonspecific, then mobile cycle arrest induced by BIM I really should not fluctuate as a operate of PTEN position. What are the implications of these observations? Amplification of EGFR has a nicely acknowledged association with innovative glioblastoma multiforme tumors. This observation, combined with the bad outcome in this disease, established higher expectations for the likely therapeutic efficacy of EGFR inhibitors in glioma. That EGFR inhibitors are of selelck kinase inhibitor constrained use clinically outcomes the two from the failure of these medicine to block PIK signaling in PTENmt tumors and from activation of multiple RTKs in glioma, creating it unlikely that blockade of any solitary RTK would final result in a long lasting medical response. The problem presented by repeated PTEN mutation merged with activation of several RTKs collectively argues for blockade of downstream signaling pathways into which these signaling inputs converge. The prominence of Akt as a signaling intermediate downstream of EGFR has generated enthusiasm for the clinical development of small molecule inhibitors of Akt. We were thus astonished to find that inhibition of Akt activation could be selleck chemical 10058-F4 reached in PTENmt glioma with dosages of erlotinib that unsuccessful to impact proliferation. We confirmed even further that neither blockade nor activation of Akt impacted proliferation or reaction to erlotinib in glioma.
zhazha is offline  
Related Topics
Thread Starter
Last Post
General Forum
03-20-2013 03:03 AM
02-03-2006 06:47 PM

Currently Active Users Viewing This Thread: 1 (0 members and 1 guests)

Thread Tools
Search this Thread
Quick Reply: Interesting Twitter Posts Regarding Inhibitors